INDIGO Biosciences is offering one free Luciferase Detection Reagent Kit (LDR-10) - the same luciferase detection reagent that enhances the sensitivity of its ready-to-use reporter assays - for new users to try when purchasing through Scientist.com. This offer is valid from March 1st until May 31st, 2024.

Luciferase Detection Reagent Kit

Features Contents Ready-to-use INDIGlo LDR, formulated as a 2x-concentrated reagent Stable light emission for over 2 hours Dilution Buffer, for optional use if performing non-homogenous assays Rapid lysis and robust signal 2x-concentrated reagent Suitable for 96- or 384-well format assays

INDIGO’s INDIGlo Luciferase Detection Reagent is formulated to deliver steady, long-lived luminescence signal from mammalian reporter cells expressing firefly luciferase. For convenience, the reagent is provided ‘ready to use’, eliminating the need for reconsitution of powder-form components, ensuring seamless integration into laboratory workflows.

Designed for versatility, INDIGlo Luciferase Detection Reagent (LDR) performs optimally in both 96- or 384-well format reporter assays, providing stable light emission over a 2-hour period. This stability makes it ideal for economical detection in both low- and high-throughput batch processing of assay plates.

The 2x-concentrated formulation of INDIGlo LDR offers enhanced flexibility, allowing for optimal performance in various assay setups. Whether using 384-well or 1536-well assay plates with a homogenous assay strategy or 96-well assay plates with either homogenous or non-homogenous assay strategies, INDIGlo LDR ensures rapid lysis of reporter cells, robust luminescence signals, and sensitive quantification of luciferase activities.

INDIGlo LDR performance from both Homogenous and Non-Homogenous assays. Dose-response analyses of Human AhR (INDIGO #IB06001, 96-well assay) was performed using the reference agonist MeBIO. AhR reporter cells were plated across two white assay plates, MeBIO treatment media were added, and the assay plates incubated for 24 hr. Plates were then processed with luciferase detection reagents in either a.) a homogenous or b.) a non-homogenous assay format. Resulting assay metrics are also shown from using another extended-glo detection reagent (Vendor X).

INDIGO Biosciences specializes in ready-to-use cell-based reporter assays and other innovative in vitro solutions

Founded in 2005, INDIGO Biosciences began by providing contract services for the in vitro screening of small molecule compounds against human and non-human nuclear receptors. Through groundbreaking innovations such as CryoMite™, an exclusive cryo-preservation technique for reporter cells, INDIGO has evolved to develop an extensive portfolio of assay kits, enabling clients to conduct robust cell-based reporter assays in their own laboratories. Committed to innovation, INDIGO Biosciences continues to pioneer new assays and enhance existing ones to meet the evolving needs of bioscience research globally.