Beyond kinases and epigenetic enzymes, the assay menu reaches phosphatases, phosphodiesterases, kinesin ATPases, isocitrate dehydrogenase, PARP, and the ubiquitin-proteasome and heat shock pathways. Binding questions are answered by SPR, thermal shift, microscale thermophoresis, and isothermal titration calorimetry, while LinkLight reports protein-protein interactions and NanoBRET measures intracellular target engagement. Phenotypic work runs through ProLiFiler cancer cell panels in monolayer and 3D spheroid formats, plus soft agar, migration, and angiogenesis assays. In vivo pharmacology covers subcutaneous, orthotopic, and metastatic xenografts, a hollow fiber model, and preclinical irradiation, with immuno-oncology programs built on CAR-T platforms, humanized xenograft and syngeneic mice, SubQperior tumor models, and mouse-derived isografts. Cardiac safety assessment, cytochrome P450 assays, GLP regulatory toxicology, and GMP bioassay and potency testing carry the same chemistry into regulated stages.
Reaction Biology
Reaction Biology is a premier provider of drug discovery services, specializing in high-throughput screening, kinase profiling, biophysical assays, and cell-based oncology models. Leveraging one of the industry's largest collections of active enzymes, Reaction Biology delivers robust biochemical and epigenetic data to accelerate small molecule lead optimization.
