Peter Delaney reviews current trends and technological advancements in the field of in vivo fluorescence imaging, with a focus on new applications and capabilities as realized by confocal fluorescence endomicroscopy and 3D automated optical sectioning.

In vivo fluorescence microscopy is advancing rapidly with the development of new imaging modalities and fluorescence labeling agents. It is now possible to image morphological, molecular, genetic, pathological and physiological events in the living animal. Laser scanning microscopes have advanced quickly, and even miniaturized confocal microscopes of endoscopic proportions can now create 3D observations at the cellular and sub-cellular level in vivo. This has led to a proliferation of techniques facilitating the transition of therapeutic research, bench-to-bedside, through direct in vivo observation of systems biologic processes.

In this exclusive webinar sponsored by Scintica Instrumentation, Peter Delaney discusses unique combinations of advanced imaging instrumentation, fluorescent probes and key applications in terms of their capabilities, limitations and possibilities. Discussion centers on the importance of sub-cellular and cellular observations in vivo, often producing results counter to in vitro cellular behavior, and therefore streamlining research paths. In addition, he shares a variety of sample images, demonstrating novel capabilities in the world of high-resolution in vivo confocal endomicroscopy.