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Best Practices: Measuring Force, Resting Sarcomere Length, and Calcium in Cardiac Slice Preparations

Best Practices: Measuring Force, Resting Sarcomere Length, and Calcium in Cardiac Slice Preparations

Webinar Summary

How to maximize force development in cardiac slices.
How and why to acquire resting sarcomere length.
How to best load calcium indicators in cardiac slice and optimizing fluorescence signal-to-noise ratio (SNR).

Unlike isolated cardiomyocytes, cardiac tissue slices better preserve native myocardial tissue, including both fibroblasts and myocytes. And unlike whole heart studies, slices are better suited for acquisition of functional parameters like calcium transients. Unlike both models, however, cardiac slices have not been widely utilized.

This demo is exclusively available to those who attend live.

Presenters

Adam Veteto

IonOptix/University of Missouri (Medical Pharmacology and Physiology)

Applications Scientist/Visiting Scientist

Dr. Adam Veteto joined IonOptix as an Applications Scientist in 2019 after completing his PhD in Medical Pharmacology and Physiology at the University of Missouri. He continues collaborating with investigators at MU to elucidate mechanisms underlying changes in cardiac calcium homeostasis with aging due to mechanical perturbation.

IonOptix

IonOptix is passionate about providing innovative research solutions for high speed quantitative fluorescence, muscle mechanics and tissue engineering.

Topics

Disease Models

Bioimaging

Best Practices: Measuring Force, Resting Sarcomere Length, and Calcium in Cardiac Slice Preparations

Webinar Summary

Presenters

Adam Veteto

Sponsor

IonOptix

Topics

Register Now

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