Webinar Summary
- Acquiring work loops in cardiac slices
- Visualizing the Frank-Starling relationship in cardiac slices
- Using cardiac slices to understand the molecular mechanisms responsible for cardiac stiffening
- How to acquire work loops in engineered heart tissue
Cardiac slices better preserve the structure, function, and biochemical properties of the in situ heart when compared to other model systems, including isolated cardiac myocytes and other intact tissue preparations, while allowing for a wider range of experimentation than whole heart. Additionally, cardiac slices can be prepared from both animal and human tissue, suggesting that data will translate better from the bench to the clinic.
In this webinar, the speakers build off the previous IonOptix webinar introducing the cardiac slice preparation to explore specific applications that are well-suited to cardiac slices and the IonOptix Cardiac Slice System. Specifically, they show investigations utilizing cardiac slices to highlight the effect of myosin ATPase inhibition on the Frank-Starling relationship, as well as the relationship between microtubule network remodeling and diastolic stiffness/dysfunction. Lastly, they also explore engineered heart tissue as an alternative to the cardiac slice preparation.
Presenters
Bradley Palmer
Dr. Bradley Palmer's interests have focused on bringing engineering principles to combat heart failure. His most recent work has focused on using cardiac slices mimicking the Pressure-Volume relationship to detect and discern the molecular mechanisms responsible for cardiac function observed with pathological states like heart failure, diabetes, and hypertension.
Matthew Caporizzo
Dr. Matthew Caporizzo's Lab studies the mechanics of heart failure with particular emphasis on understanding the molecular mechanisms that stiffen the heart and how the heart's stiffness influences its function in health and heart disease.
Benjamin Lee
Dr. Benjamin Lee is currently a cardiology fellow at the University of Pennsylvania. He received his MD/PhD from Columbia University in biomedical engineering. He is fascinated by beating heart tissues and is using 3-dimensional systems to better understand and target mechanical dysfunction in the heart.
Sponsor
IonOptix
Content Partners
American Physiological Society
About APS
Mission: To advance scientific discovery, understand life, and improve health. Vision: A global multidisciplinary community of scientists solving the major problems affecting life and health. Founded in 1887, the American Physiological Society is a global leader in expanding knowledge related to biological function. We connect a multidisciplinary community of nearly 10,000 scientists and educators from around the world, driving collaboration and spotlighting scientific discoveries in physiology and related disciplines. Our members are advancing treatments and cures for everything from cancer and heart disease, to obesity and addiction. They are also deepening our insight into living organisms generally, helping us to better understand how things like climate change are affecting the world around us. The Society serves this dynamic community in many ways, including:- Publishing 16 globally recognized scholarly journals and a highly-regarded book monograph series;
- Sponsoring scientific meetings and symposia that explore the frontiers of bio-medical science;
- Advocating for science-smart public policy that benefits both our members and the public;
- Bestowing hundreds of awards, grants, and fellowships that recognize scientific excellence and promise;
- Championing physiology education and educators, to support bigger discoveries and better clinical outcomes;
- And providing career resources and other career services, ensuring that all our members have a pathway to success.
