CAR-T cell therapy, a groundbreaking cancer treatment, has a history of over 60 years of research. It originated from the discovery of bone marrow transplantation in the 1950s and the use of antibodies as guides for T cells in the early 1900s. The development of chimeric antigen receptors (CARs) in 1987 paved the way for CAR-T cells to target tumors. In 2017, historic approvals were granted for CAR-T therapies, including Tisagenlecleucel and Axicabtagene ciloleucel. Since then, several CAR-T cell therapies have achieved worldwide approvals, revolutionizing cancer treatment. Despite challenges, CAR-T therapy shows immense potential for improving human health.

Sino Biological offers a range of fluorescent-labeled target proteins that can be directly used in CAR detection, providing a more efficient method compared to secondary antibody cross-reactions that can cause background staining. These fluorescent-labeled proteins have high bio-activity, good uniformity, and high batch-to-batch consistency, making them ideal for CAR-T research. Sino Biological’s protein expression platform allows for customized services, including multiple labeling approaches such as FITC, PE, APC, and other fluorescent dyes.

In addition, Sino Biological has developed pre-labeled and experimentally validated biotinylated recombinant proteins. These proteins feature biotinylation at a single lysine residue within the Avi-tag, a 15 amino acid peptide. This approach ensures that the protein’s bioactivity remains unchanged, eliminating any interference. The binding between biotin and streptavidin/avidin (SA) is highly specific and sensitive, making it an excellent tool for detecting the positive rate of CAR expression. This eliminates the issues of heterogeneity and impaired function often associated with chemical biotinylation.

Overall, Sino Biological provides a comprehensive solution for CAR-T quality control, offering fluorescent-labeled target proteins and biotinylated recombinant proteins that facilitate efficient and accurate detection of CAR expression.

Below are a list of fluorescent labelled, biotin labelled, and unconjugated proteins.

Fluorescent-Labeled Target Proteins

Cat# Protein Species Conjugate Expression host Tag 10620-H03H-P BCMA Human PE HEK293 His & hFc 12238-HCCH-P CD33 Human PE HEK293 - 10818-H08H-P CD38 Human PE HEK293 His

Biotin-labeled Target Proteins

Cat# Protein Species Expression host Tag Purity 10818-H27H-B CD38 Human HEK293 His & AVI >95% 10673-H27H-B NCAM1 Human HEK293 His & AVI >95% 29662-H27H-B EGFR Human HEK293 His & AVI >95% 10679-H27H-B ULBP1 Human HEK293 His & AVI >95% 12143-H27H-B ULBP2 Human HEK293 His & AVI >95% 13926-H27H-B EPHA2 Human HEK293 His & AVI >95% 10692-H27H-B c-MET Human HEK293 His & AVI >95% 11027-H27H-B CD5 Human HEK293 His & AVI >95% 11958-H41H-B CD22 Human HEK293 hFc & AVI >90% 10350-H08H-B IL13RA2 Human HEK293 His >95% 10012-H08H-B VEGFR2 Human HEK293 His >95% 11077-H08H-B CEACAM5 Human HEK293 His >95% 11880-H08H-B CD19 Human HEK293 His >90% 12238-H08H-B CD33 Human HEK293 His >90% 10777-H08H-B CD30 Human HEK293 His >90% 29662-H08B-B EGFR Human Baculovirus-Insect His >90% 15877-H07H-B PSMA Human HEK293 His >90% 10088-H08H-B Glypican-3 Human HEK293 His >85% 10464-H07H-B FAP Human HEK293 His >85% 10084-H02H-B PD-L1 Human HEK293 hFc >95% 10694-H02H-B EpCAM Human HEK293 hFc >90% 13128-H01H-B Mesothelin Human HEK293 hFc >85% 11007-H34E-B CD20 Human E. coli TrxA >85% 13968-HCCH1-B ROR1 Human HEK293 - >95% 10004-HCCH-B HER2 Human HEK293 - >95%

Unconjugated Target Proteins

Cat# Protein Species Expression host Tag Purity Bioactivity 11880-H08H CD19 Human HEK293 C-His >90% active 11958-h08h CD22 Human HEK293 C-His >95% active 11958-HNAH CD22 Human HEK293 Native >95% - 10777-H08H CD30 Human HEK293 C-His >95% active 10818-H32H CD38 Human HEK293 N-His & FLAG >93% - 10012-h02h1 VEGFR2 Human HEK293 C-human IgG1-Fc >95% active 13926-h20b1 EPHA2 Human Baculovirus-Insect N-GST & His >95% active 11028-h08h CD7 Human HEK293 C-His >95% active 29662-h02b EGFR Human Baculovirus-Insect C-human IgG1-Fc >95% active 30025-hnah CLDN18 Human HEK293 Native >90% active 10088-h08h Glypican-3 Human HEK293 C-His >87% active 10692-H20B1 c-MET Human Baculovirus-Insect Cells N-GST & His >90% active 10107-h08h CA9 Human HEK293 C-His >95% active 10084-h05h PD-L1 Human HEK293 C-mouse IgG1-Fc >95% active 10084-HNAH PD-L1 Human HEK293 Native >95% - 10121-H02H IL1RAP Human HEK293 C-human IgG1-Fc >95% - 10518-H02H CD123 Human HEK293 C-human IgG1-Fc >95% - 10377-H08H PD-1 Human HEK293 C-His >95% active 10679-h03h ULBP1 Human HEK293 C-human IgG1-Fc & His >95% active 12143-H08H ULBP2 Human HEK293 C-His >97% - 15877-h07h PSMA Human HEK293 N-His >95% active