This blog post was written by Ignyte Bio. Ignyte Bio, headquartered in Seattle, WA, specializes in providing researchers with high-quality, well-characterized immune cells through their unique “4R” model. This model emphasizes accessible product data, recallable donors, instant purchasing and ready-to-use cell products, all aimed at enhancing transparency and efficiency in cell sourcing. Their offerings include a broad range of cryopreserved human PBMCs, isolated immune cell components, and Unique immune cells like Antigen Specific T cells, induced TRegs and Expanded NK cells. Their services are available on Scientist.com.

What are the “4R” Solutions to Cell Sourcing?

Readily Available Data: Each product comes with donor and lot-specific data, accessible via certificates of analysis posted on the Ignyte Bio website for thorough review.

Recallable Donors: Donors can be recalled at no extra cost, supporting reproducibility and further studies.

Ready for Purchase: Real-time inventory is maintained on the website, allowing for immediate purchase and streamlined ordering.

Ready to Use: Products include lot-specific phenotypic and functional data, enabling researchers to select the optimal cell products for their specific assays.

Available Cell Types

Ignyte Bio offers a diverse selection of cryopreserved human peripheral blood mononuclear cells (PBMC), their isolated components and in vitro generated cell types, including:

T Cells ( CD3 , CD4 , CD8 ) Monocytes B Cells Monocyte-derived Dendritic Cells (DC) Isolated NK Cells B-LCL

Specialized Immune Cell Products

Antigen-Specific T Cells (ASpecT)

Produced through multiple in vitro stimulations using peptide antigens.

Not immortalized or genetically modified, closely mimicking physiological T cells.

Specificity is verified via cognate peptide/MHC tetramer binding and interferon-gamma (IFN-γ) secretion assays.

Applications: Assessing antigen presentation (such as vaccine potency) Evaluating factors that modulate T cell responses, including: cytokines agents targeting co-stimulatory molecules checkpoint molecules.

ASpecT Product Line ( CD4 and CD8 )

Anti-gp100 CD8 T Cells Anti-MUC1 CD8 T Cells Anti-CMV CD8 T Cells Anti-HPV E7 CD8 T Cells Anti-CEA CD8 T Cells Anti-GAD65 CD4 T Cells Anti-Tyrosinase CD8 T Cells Anti-PSMA CD8 T Cells Anti-GAD65 CD4 T Cells Anti-MART-1 CD8 T Cells Anti-NYESO CD8 T Cells Anti-NY-ESO-1 CD4 T Cells

Induced Regulatory T Cells (Treg)

In vitro generation of iTregs allows for cost-effective, larger batch production, enhancing their applicability in immunology and oncology research.

Ideal for Treg suppression assays, facilitating the evaluation of drug candidates in suppressing or inhibiting immune responses.

Each lots exhibit induced Treg phenotypes: CD4+, CD25+, CD127 low/negative, significant Foxp3 expression, and a proven capacity to suppress T cell proliferation.

Expanded NK Cells

Offered at a lower price point and in larger lot sizes compared to traditional NK cell isolation methods.

High viability (>85%) and purity (>85%) for the CD56+, CD3- population.

Comprehensive donor information, including FcγRIIIA-158 genotype, is included in each certificate of analysis.

Cells are functional and ready for use immediately after thawing, with proven activity in direct cell killing assays (e.g., K562) and antibody-dependent cellular cytotoxicity (ADCC) assays. The figure below shows comparison of ADCC mediated by isolated vs expanded NK cells from the same donor

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